Moretti V M, van de Water C, Haagsma N
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, University of Utrecht, Netherlands.
J Chromatogr. 1992 Nov 27;583(1):77-82. doi: 10.1016/0378-4347(92)80346-r.
An on-line high-performance liquid immunoaffinity chromatographic (HPLIAC) system for the direct determination of chloramphenicol in milk and swine muscle tissue is described. The system consisted of a dual-column system in which an HPLIAC column was directly coupled to an RP-8 high-performance liquid chromatographic column. Skimmed and deproteinated milk or aqueous muscle tissue extract was directly injected into the HPLIAC column. After a washing step with phosphate-buffered saline, chloramphenicol was desorbed by a glycine-NaCl buffer (pH 2.8) and directly concentrated on the RP-8 column. Next, chromatography was carried out using acetonitrile-sodium acetate buffer as the mobile phase. Chloramphenicol was detected at 280 nm. Mean recoveries from spiked raw milk were 70 +/- 2% (1-50 micrograms/kg) and from spiked swine muscle tissue 64 +/- 2% (10-50 micrograms/kg). The calibration curves were linear in the range 1-200 micrograms/kg spiking levels. Limits of determination were 1 microgram/kg for milk and 10 micrograms/kg for muscle tissue.
描述了一种用于直接测定牛奶和猪肌肉组织中氯霉素的在线高效液相免疫亲和色谱（HPLIAC）系统。该系统由双柱系统组成，其中HPLIAC柱直接与RP - 8高效液相色谱柱相连。脱脂和脱蛋白的牛奶或肌肉组织水提取物直接注入HPLIAC柱。用磷酸盐缓冲盐水洗涤后，用甘氨酸 - 氯化钠缓冲液（pH 2.8）解吸氯霉素，并直接浓缩在RP - 8柱上。接下来，使用乙腈 - 醋酸钠缓冲液作为流动相进行色谱分析。在280nm处检测氯霉素。加标原料奶的平均回收率为70±2%（1 - 50微克/千克），加标猪肌肉组织的平均回收率为64±2%（10 - 50微克/千克）。在1 - 200微克/千克的加标水平范围内校准曲线呈线性。牛奶的测定限为1微克/千克，肌肉组织的测定限为10微克/千克。
