Gude T, Preiss A, Rubach K
Bundesinstitut für gesundheitlichen Verbraucherschutz, Berlin, Germany.
J Chromatogr B Biomed Appl. 1995 Nov 17;673(2):197-204. doi: 10.1016/0378-4347(95)00280-5.
A rapid and specific clean-up procedure based on immunoaffinity chromatography (IAC) with polyclonal antibodies for the gas chromatographic determination with electron-capture detection of chloramphenicol in pig muscle tissue, organs and urine is described. A commercially available IAC material was used for the analysis. A decrease in the capacity of the column after being used more than 100 times was observed. Mean recoveries were 69, 54, 62 and 95% for spiked pig muscle tissue, liver, kidney and urine, respectively. The limit of detection was 0.2 micrograms/kg for muscle tissue, 2.0 micrograms/kg for liver and kidney and 0.4 micrograms/kg for urine.
本文描述了一种基于免疫亲和色谱（IAC）和多克隆抗体的快速、特异性净化程序，用于猪肌肉组织、器官和尿液中氯霉素的气相色谱电子捕获检测。分析使用了市售的IAC材料。观察到该柱使用100次以上后容量下降。加标猪肌肉组织、肝脏、肾脏和尿液的平均回收率分别为69%、54%、62%和95%。肌肉组织的检测限为0.2微克/千克，肝脏和肾脏为2.0微克/千克，尿液为0.4微克/千克。
