Kajiwara K, Fukushima H, Kishikawa H, Nishida K, Hashiguchi Y, Sakakida M, Uehara M, Shichiri M
Department of Metabolic Medicine, Kumamoto University Medical School, Japan.
Med Prog Technol. 1992;18(3):181-9.
As an alternative way of long-term glycemic monitoring, the glucose measurement by analyzing Fourier transform infrared absorbance spectra with an attenuated total reflection prism has been developed. In glucose aqueous solution, glucose has characteristic absorptions at the wave numbers of 1033 and 1080 cm-1 and the absorption intensities are proportional to glucose concentrations. In serum and whole blood samples, however, red blood cell corpuscles, serum albumin and serum gamma-globulin interfere with the absorbance spectra of glucose and shift the base line upward significantly. Therefore, to eliminate these interferences in serum and whole blood samples, the feasibility of the calibration curves obtained by using difference absorbance spectra with those of fasting samples was studied. As a result, highly significant correlations between glucose concentrations estimated by Fourier transform infrared spectroscopic method and those measured by glucose oxidase method were obtained (r = 0.981 and 0.989 for serum and whole blood samples, respectively). From these experiments, it was concluded that by infrared spectroscopy glucose concentrations in the serum and whole blood samples could be measured quantitatively or monitored if the base line drifts and interferences were subtracted.
作为长期血糖监测的一种替代方法，通过用衰减全反射棱镜分析傅里叶变换红外吸收光谱来测量葡萄糖的方法已被开发出来。在葡萄糖水溶液中，葡萄糖在波数1033和1080 cm-1处有特征吸收，且吸收强度与葡萄糖浓度成正比。然而，在血清和全血样本中，红细胞、血清白蛋白和血清γ-球蛋白会干扰葡萄糖的吸收光谱，并使基线显著上移。因此，为消除血清和全血样本中的这些干扰，研究了使用与空腹样本的吸收光谱差值得到的校准曲线的可行性。结果，通过傅里叶变换红外光谱法估算的葡萄糖浓度与用葡萄糖氧化酶法测量的葡萄糖浓度之间获得了高度显著的相关性（血清和全血样本的r分别为0.981和0.989）。从这些实验得出结论，通过红外光谱法，如果减去基线漂移和干扰，血清和全血样本中的葡萄糖浓度可以进行定量测量或监测。
