使用溶蚀性微针进行皮肤间质液中万古霉素的治疗药物监测。
Therapeutic Drug Monitoring of Vancomycin in Dermal Interstitial Fluid Using Dissolving Microneedles.
作者信息
Ito Yukako, Inagaki Yuto, Kobuchi Shinji, Takada Kanji, Sakaeda Toshiyuki
机构信息
1. Department of Pharmacokinetics, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto, 607-8412, Japan.
2. BioSerenTach Inc., Shimogyo-ku, Kyoto, 600-8040, Japan.
出版信息
Int J Med Sci. 2016 Mar 16;13(4):271-6. doi: 10.7150/ijms.13601. eCollection 2016.
OBJECTIVE
To design an alternative painless method for vancomycin (VCM) monitoring by withdrawing interstitial fluid (ISF) the skin using dissolving microneedles (DMNs) and possibly replace the conventional clinical blood sampling method.
METHODS
Male Wistar rats were anesthetized with 50 mg/kg sodium pentobarbital. Vancomycin at 5 mg/mL in saline was intravenously administered via the jugular vein. ISF was collected from a formed pore at 15, 30, 45, 60, 75, 90, and 120 min after the DMNs was removed from the skin. In addition, 0.3 mL blood samples were collected from the left femoral vein.
RESULTS
The correlation between the plasma and ISF VCM concentrations was significantly strong (r = 0.676, p < 0.05). Microscopic observation of the skin after application of the DMNs demonstrated their safety as a device for sampling ISF.
CONCLUSION
A novel monitoring method for VCM was developed to painlessly determine concentrations in the ISF as opposed to blood sampling.
目的
设计一种替代方法，通过使用溶解微针（DMN）从皮肤抽取组织间液（ISF）来进行万古霉素（VCM）监测，并可能取代传统的临床血液采样方法。
方法
用50mg/kg戊巴比妥钠麻醉雄性Wistar大鼠。通过颈静脉静脉注射5mg/mL生理盐水的万古霉素。在DMN从皮肤移除后15、30、45、60、75、90和120分钟，从形成的孔中收集ISF。此外，从左股静脉采集0.3mL血样。
结果
血浆和ISF中VCM浓度之间的相关性显著强（r = 0.676，p < 0.05）。应用DMN后对皮肤的显微镜观察证明了其作为ISF采样装置的安全性。
结论
开发了一种新的VCM监测方法，与血液采样不同，该方法可无痛测定ISF中的浓度。
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