Dissanayake S, Xu M, Piessens W F
Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115.
Mol Biochem Parasitol. 1992 Dec;56(2):259-67. doi: 10.1016/0166-6851(92)90175-j.
We have isolated and characterized a gene encoding a novel GTP-binding protein of the GTPase superfamily in the filarial parasites Brugia malayi and Onchocerca volvulus. The deduced amino acid sequence of the cloned molecule has approximately 30% overall homology to ras proteins and approximately 90% homology to the 'ras-like' nuclear proteins TC4, ran and Spil. Rabbit antisera to bacterially expressed filarial protein detect a 24-22 kDa doublet in extracts of adult B. malayi and mature microfilariae, which is absent from immature microfilariae. Increased expression of the native parasite protein occurs when worms are cultured in the presence of epidermal growth factor.
我们已经从丝虫马来布鲁线虫和盘尾丝虫中分离并鉴定了一个编码GTP酶超家族新型GTP结合蛋白的基因。克隆分子推导的氨基酸序列与ras蛋白总体上约有30%的同源性，与“类ras”核蛋白TC4、ran和Spil约有90%的同源性。针对细菌表达的丝虫蛋白的兔抗血清在成年马来布鲁线虫和成熟微丝蚴的提取物中检测到一个24 - 22 kDa的双峰，而未成熟微丝蚴中没有。当蠕虫在表皮生长因子存在的情况下培养时，天然寄生虫蛋白的表达会增加。
