Neumann S, Ziv E, Lantner F, Schechter I
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Mol Biochem Parasitol. 1992 Dec;56(2):357-60. doi: 10.1016/0166-6851(92)90188-p.
Schistosomes have a complex life cycle (vertebrate and molluscan hosts as well as larvae living freely in water) in which they are exposed to different environments and temperatures (20 degrees C - 37 degrees C). Since heat shock genes are activated in response to stress and during development [1], it is of interest to study the hsp70 gene family in schistosome. To approach this issue we have isolated from Schistosoma mansoni a genomic clone containing the complete coding region of hsp70 and the 5' flanking DNA with transcription regulatory elements including HSE (heat shock element) sequences.
血吸虫具有复杂的生命周期（包括脊椎动物宿主、软体动物宿主以及在水中自由生活的幼虫），在此过程中它们会接触到不同的环境和温度（20摄氏度 - 37摄氏度）。由于热休克基因在应激反应和发育过程中被激活[1]，因此研究血吸虫中的hsp70基因家族很有意义。为了解决这个问题，我们从曼氏血吸虫中分离出一个基因组克隆，该克隆包含hsp70的完整编码区以及带有转录调控元件（包括热休克元件（HSE）序列）的5'侧翼DNA。
