克隆编码曼氏血吸虫亲环蛋白p50的基因。

Cloning the gene encoding Schistosoma mansoni p50, an immunophilin.

作者信息

Kiang D, Karim A M, LoVerde P T

机构信息

Department of Microbiology, State University of New York, Buffalo 14214, USA.

出版信息

Gene. 1996 Apr 17;170(1):137-40. doi: 10.1016/0378-1119(95)00829-2.

DOI:10.1016/0378-1119(95)00829-2

PMID:8621074

Abstract

A 2.2-kb fragment of genomic DNA encoding Schistosoma mansoni immunophilin p50 (Smp50) was identified on a 14-kb genomic clone. The sequence of Smp50 reveals seven exons interrupted by six small introns ranging from 28-35 bp in size. The transcription start point, defined by primer extension analysis of schistosome RNA, begins at 30 bp upstream from the start AUG codon. Smp50 lacks a TATA box and appears to be a single-copy gene.

摘要

在一个14kb的基因组克隆上鉴定出一段编码曼氏血吸虫亲免蛋白p50（Smp50）的2.2kb基因组DNA片段。Smp50的序列显示有7个外显子，被6个大小在28 - 35bp的小内含子打断。通过对血吸虫RNA进行引物延伸分析确定的转录起始点，位于起始AUG密码子上游30bp处。Smp50缺乏TATA框，似乎是一个单拷贝基因。