Hulsey M G, Martin R J
Department of Foods and Nutrition, College of Family and Consumer Sciences, University of Georgia, Athens 30602.
Physiol Behav. 1992 Dec;52(6):1141-9. doi: 10.1016/0031-9384(92)90473-f.
Parabiosis and blood-transfer studies with rodents suggest the existence of humoral factors capable of affecting energy balance. The nature and origin of these factors is undetermined. Aqueous extracts of adipose tissue from overfed rats significantly reduce food intake when administered intraperitoneally (IP) or intracerebroventricularly (ICV). We term the agent(s) responsible for this effect adipose satiety factor (ASF). A single IP dose of ASF, equivalent to 44 mg crude protein, suppresses cumulative food intake for over 12 h. ASF, prepared using a combination of adipose tissue from obese Zucker rats and overfed rats, is more potent per unit of protein than ASF prepared exclusively using adipose tissue from overfed rats. A single ICV dose of this hybrid preparation, equivalent to 14.6 micrograms of crude protein, suppresses cumulative food intake by 40% for up to 48 h. By ultrafiltration, the molecular weight associated with maximal ASF activity is between 30 and 100 kilodaltons (kDa). The behavioral specificity of ASF-induced anorexia is demonstrated using meal pattern, taste aversion, and differential starvation paradigms.
对啮齿动物进行的联体生活和血液转移研究表明，存在能够影响能量平衡的体液因子。这些因子的性质和来源尚未确定。给腹腔注射（IP）或脑室内注射（ICV）过量喂养大鼠的脂肪组织水提取物时，会显著减少食物摄入量。我们将导致这种效应的因子称为脂肪饱腹感因子（ASF）。单次腹腔注射相当于44毫克粗蛋白的ASF，可抑制累积食物摄入量超过12小时。使用肥胖 Zucker 大鼠和过量喂养大鼠的脂肪组织组合制备的ASF，每单位蛋白质的效力比仅使用过量喂养大鼠的脂肪组织制备的ASF更强。单次脑室内注射相当于14.6微克粗蛋白的这种混合制剂，可在长达48小时内将累积食物摄入量抑制40%。通过超滤，与最大ASF活性相关的分子量在30至100千道尔顿（kDa）之间。使用进食模式、味觉厌恶和差异饥饿范式证明了ASF诱导厌食的行为特异性。
