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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

J Gen Physiol. 1951 Jul;34(6):785-94. doi: 10.1085/jgp.34.6.785.

Simultaneous oxidation of C(14)-methyl-labeled acetate, and unlabeled malate or fumarate and alpha-ketoglutarate results in entrapment of labeled carbon in the C(4)-dicarboxylic acids, but not in alpha-ketoglutarate, although all substrates are utilized at comparable rates. 2. A large endogenous reduction of all C(4)-dicarboxylic acids (fumarate, oxalacetate, and malate) to succinate is observed under aerobic conditions, and when vigorous oxidation is proceeding. This effect occurs with both freshly harvested young (18 hour) cells and stored (2 week) cells. 3. This reduction can be considerably minimized under high oxygen tensions. 4. The quantitative concordance of these results with a Thunberg-Knoop cyclic mechanism for acetate oxidation is shown. Possible alternative C(4) products formed prior to succinate are not completely excluded, but it appears that the cells can utilize the succinate condensation as a major pathway in acetate oxidation.

对C(14) - 甲基标记的乙酸盐以及未标记的苹果酸或富马酸和α - 酮戊二酸进行同时氧化，结果是标记的碳被截留在C(4) - 二羧酸中，而不是α - 酮戊二酸中，尽管所有底物的利用速率相当。2. 在有氧条件下以及进行剧烈氧化时，观察到所有C(4) - 二羧酸（富马酸、草酰乙酸和苹果酸）大量内源性还原为琥珀酸。新鲜收获的年轻（18小时）细胞和储存（2周）的细胞都会出现这种效应。3. 在高氧张力下，这种还原作用可大大降低。4. 结果表明这些结果与乙酸盐氧化的滕伯格 - 克诺普循环机制在数量上是一致的。在琥珀酸之前形成的可能的替代C(4) 产物并未被完全排除，但似乎细胞可以将琥珀酸缩合作为乙酸盐氧化的主要途径。

J Gen Physiol. 1951 Jul;34(6):785-94. doi: 10.1085/jgp.34.6.785.

Simultaneous oxidation of C(14)-methyl-labeled acetate, and unlabeled malate or fumarate and alpha-ketoglutarate results in entrapment of labeled carbon in the C(4)-dicarboxylic acids, but not in alpha-ketoglutarate, although all substrates are utilized at comparable rates. 2. A large endogenous reduction of all C(4)-dicarboxylic acids (fumarate, oxalacetate, and malate) to succinate is observed under aerobic conditions, and when vigorous oxidation is proceeding. This effect occurs with both freshly harvested young (18 hour) cells and stored (2 week) cells. 3. This reduction can be considerably minimized under high oxygen tensions. 4. The quantitative concordance of these results with a Thunberg-Knoop cyclic mechanism for acetate oxidation is shown. Possible alternative C(4) products formed prior to succinate are not completely excluded, but it appears that the cells can utilize the succinate condensation as a major pathway in acetate oxidation.

对C(14) - 甲基标记的乙酸盐以及未标记的苹果酸或富马酸和α - 酮戊二酸进行同时氧化，结果是标记的碳被截留在C(4) - 二羧酸中，而不是α - 酮戊二酸中，尽管所有底物的利用速率相当。2. 在有氧条件下以及进行剧烈氧化时，观察到所有C(4) - 二羧酸（富马酸、草酰乙酸和苹果酸）大量内源性还原为琥珀酸。新鲜收获的年轻（18小时）细胞和储存（2周）的细胞都会出现这种效应。3. 在高氧张力下，这种还原作用可大大降低。4. 结果表明这些结果与乙酸盐氧化的滕伯格 - 克诺普循环机制在数量上是一致的。在琥珀酸之前形成的可能的替代C(4) 产物并未被完全排除，但似乎细胞可以将琥珀酸缩合作为乙酸盐氧化的主要途径。