从蓝藻水华（芬兰）中分离微囊藻毒素及其结构

Isolation and structures of microcystins from a cyanobacterial water bloom (Finland).

作者信息

Namikoshi M, Sivonen K, Evans W R, Sun F, Carmichael W W, Rinehart K L

机构信息

School of Chemical Sciences, University of Illinois, Urbana 61801.

出版信息

Toxicon. 1992 Nov;30(11):1473-9. doi: 10.1016/0041-0101(92)90523-8.

DOI:10.1016/0041-0101(92)90523-8

PMID:1485342

Abstract

A hepatotoxic cyanobacterial (blue-green algal) water bloom was collected from a constructed water reservoir in Finland. The water bloom contained two cyanobacterial species, Microcystis aeruginosa and Aphanizomenon flos-aquae. Two hepatotoxins, 1 and 2, were isolated from extracts of lyophilized cells. The structures of 1 and 2 were assigned based upon their amino acid analyses on a Waters Pico Tag HPLC system and a chiral GC capillary column (Chirasil Val III), fast atom bombardment mass spectrometry (FABMS), high resolution FABMS, and tandem FABMS data. Toxin 1 was identical to a previously reported compound, [D-Asp3]microcystin-RR. Toxin 2 was new and was assigned the structure [D-Asp3]microcystin-YR.

摘要

从芬兰一座人工蓄水池采集到一次具有肝毒性的蓝藻水华。该水华包含两种蓝藻，铜绿微囊藻和水华束丝藻。从冻干细胞提取物中分离出两种肝毒素，1和2。基于在沃特世Pico Tag高效液相色谱系统、手性气相色谱毛细管柱（Chirasil Val III）上的氨基酸分析、快原子轰击质谱（FABMS）、高分辨率FABMS以及串联FABMS数据，确定了1和2的结构。毒素1与先前报道的化合物[D-Asp3]微囊藻毒素-RR相同。毒素2是新发现的，被确定为[D-Asp3]微囊藻毒素-YR的结构。