Nilsson L A, Uggla A
Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
Vet Parasitol. 1992 Dec;45(1-2):73-80. doi: 10.1016/0304-4017(92)90028-8.
A modification of the diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) for analysis of antibodies in tissue fragments has been elaborated. In this technique, tissue specimens are placed on top of a thin gel layer covering a plastic surface coated with an antigen preparation. The gel thus serves as a medium through which diffusion of antibodies contained in tissue samples may occur. Specimens from skin, lung, liver, kidney, heart and skeletal muscle as well as blood serum and blood sampled onto filter paper were successfully used for analysis of the antibody response to two different antigens (soluble adult worm antigen and soluble egg antigen) during experimental infection of mice with the helminth parasite Schistosoma mansoni. It was concluded that skin and lung tissue reflected the serum antibody response more closely than the other tissues tested, although zone sizes were slightly smaller than those registered for corresponding serum samples. The DIG-ELISA reaction zone size was, as expected, shown to be dependent on the weight (size) of the tissue specimen. The variability of the modified technique was comparable to that found for analysis of serum by the conventional DIG-ELISA technique. It is concluded that the described technique may be used for serological analysis when blood samples cannot be easily obtained, e.g. at examination of animal carcasses or single organs.
已对用于分析组织碎片中抗体的凝胶扩散酶联免疫吸附测定法（DIG-ELISA）进行了改良。在该技术中，将组织标本置于覆盖有涂有抗原制剂的塑料表面的薄凝胶层之上。因此，凝胶充当了一种介质，组织样本中所含抗体可通过它进行扩散。来自皮肤、肺、肝、肾、心脏和骨骼肌的标本以及采集到滤纸上的血清和血液，在小鼠被曼氏血吸虫这种蠕虫寄生虫实验性感染期间，成功用于分析对两种不同抗原（可溶性成虫抗原和可溶性虫卵抗原）的抗体反应。得出的结论是，皮肤和肺组织比所测试的其他组织更能紧密反映血清抗体反应，尽管反应区大小略小于相应血清样本的反应区大小。正如预期的那样，DIG-ELISA反应区大小显示取决于组织标本的重量（大小）。改良技术的变异性与通过传统DIG-ELISA技术分析血清时的变异性相当。得出的结论是，当不易采集血样时，例如在检查动物尸体或单个器官时，所描述的技术可用于血清学分析。
