Nakai Y, Uchida T, Kanazawa K
Department of Animal Science, Ibaraki University, Japan.
Avian Dis. 1992 Oct-Dec;36(4):1034-6.
Immunization of chickens was attempted with low levels of Eimeria tenella oocysts (50 oocysts per day) over the first 1 or 2 weeks of life--the "trickle infection" (TI) method. When chickens were immunized by TI at 0-13 days of age, no cecal lesions and a reduced number of oocysts in ceca were observed after challenge at 17 days of age. TI at 0-6 days of age conferred better protection against challenge with E. tenella than did TI at 7-13 days. However, cecal lesions were observed in almost all of these chickens. These findings indicated that TI for 2 weeks (0-13 days of age) provided better immunity than TI for 1 week (0-6 or 7-13 days of age).
在雏鸡生命的最初1或2周内，尝试用低水平的柔嫩艾美耳球虫卵囊（每天50个卵囊）对其进行免疫——即“微量感染”（TI）法。当雏鸡在0至13日龄时通过微量感染进行免疫，在17日龄受到攻击后，未观察到盲肠病变，且盲肠中的卵囊数量减少。0至6日龄时进行微量感染比7至13日龄时进行微量感染对柔嫩艾美耳球虫攻击的保护效果更好。然而，几乎所有这些雏鸡都观察到了盲肠病变。这些结果表明，2周（0至13日龄）的微量感染比1周（0至6日龄或7至13日龄）的微量感染提供了更好的免疫力。
