Hamlin J L
Department of Biochemistry, University of Virginia, Charlottesville 22908.
Crit Rev Eukaryot Gene Expr. 1992;2(4):359-81.
Mammalian origins of replication are scattered at about 100-kb intervals along each chromosomal DNA fiber. It is not known whether origins are genetically defined sequences analogous to those that control initiation of replication in microorganisms. Since the advent of recombinant DNA techniques, it has become possible to search for origins in defined chromosomal domains. A variety of methods have suggested that nascent chains initiate at preferred locations on the chromosome, but there is some disagreement as to how precisely defined these initiation sites are.
哺乳动物的复制起点沿着每条染色体DNA纤维以大约100千碱基对的间隔分散分布。目前尚不清楚这些起点是否是类似于控制微生物复制起始的那些基因定义序列。自重组DNA技术问世以来，在特定染色体区域中寻找复制起点已成为可能。多种方法表明新生链在染色体上的优先位置起始，但对于这些起始位点的精确定义程度存在一些分歧。
