Vassilev L T, DePamphilis M L
Department of Cell and Developmental Biology, Roche Research Center, Nutley, NJ 07110.
Crit Rev Biochem Mol Biol. 1992;27(6):445-72. doi: 10.3109/10409239209082569.
Several experimental approaches for identification of origins of DNA replication have been developed recently that allow, for the first time, unique initiation sites in mammalian chromosomes to be mapped at single-copy loci. A brief description of the rationale, advantages, and limitations has been provided for each approach, as well as information that can help the reader choose the method(s) most suitable for a particular system. The various methods are divided into three groups: (1) analysis of nascent DNA strands, (2) analysis of DNA structures, and (3) analysis of origin activity (i.e., ability to support autonomous replication). It is hoped that this information will serve as a practical guide for identifying new origins of replication.
最近已经开发出几种用于鉴定DNA复制起点的实验方法，这些方法首次能够在单拷贝基因座上绘制哺乳动物染色体中的独特起始位点。已针对每种方法提供了原理、优点和局限性的简要描述，以及有助于读者选择最适合特定系统的方法的信息。各种方法分为三组：（1）新生DNA链分析，（2）DNA结构分析，（3）起始活性分析（即支持自主复制的能力）。希望这些信息将作为鉴定新复制起点的实用指南。
