Karmali R, Schiffmann S N, Vanderwinden J M, Hendy G N, Nys-DeWolf N, Corvilain J, Bergmann P, Vanderhaeghen J J
Laboratory of Experimental Medicine, Brugmann Hospital, Brussels, Belgium.
Cell Tissue Res. 1992 Dec;270(3):597-600. doi: 10.1007/BF00645063.
Previous studies have indicated that 19-day-old fetal long bones of the rat contain an adenylyl cyclase-stimulating activity antigenically related to parathyroid hormone-related peptide. To ascertain its origin, Northern blotting and in situ hybridization histochemistry were performed. Results demonstrate that mRNA of parathyroid hormone-related peptide is present in RNA extracted from fetal long bones of the rat and that cells responsible for its production are localized in the periosteum. These cells are not mature osteoblasts because they do not synthesize mRNA of osteocalcin. Thus the present study shows that parathyroid hormone-related peptide could be produced locally, at least in part, in the skeleton of fetal rats.
以往的研究表明，19日龄大鼠胎儿的长骨含有一种与甲状旁腺激素相关肽抗原性相关的腺苷酸环化酶刺激活性。为确定其来源，进行了Northern印迹法和原位杂交组织化学分析。结果表明，甲状旁腺激素相关肽的mRNA存在于从大鼠胎儿长骨提取的RNA中，且产生该肽的细胞定位于骨膜。这些细胞不是成熟的成骨细胞，因为它们不合成骨钙素的mRNA。因此，本研究表明甲状旁腺激素相关肽至少部分可在胎鼠骨骼中局部产生。
