醇链长度对1,2-双(10,12-二十三碳二炔酰基)-sn-甘油-3-磷酸胆碱中肾小管形成的影响。

Effect of alcohol chain length on tubule formation in 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine.

作者信息

Ratna B R, Baral-Tosh S, Kahn B, Schnur J M, Rudolph A S

机构信息

Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington DC 20375-5320.

出版信息

Chem Phys Lipids. 1992 Nov;63(1-2):47-53. doi: 10.1016/0009-3084(92)90021-g.

DOI:10.1016/0009-3084(92)90021-g

PMID:1486660

Abstract

Aqueous dispersions of 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine, on cooling below the chain melting temperature, form hollow cylindrical structures known as 'tubules'. We have studied the formation of tubules in methanol/water, ethanol/water and n-propanol/water. For each alcohol, there is a defined window of alcohol/water ratios in which the lipid precipitates with the tubule morphology. As the chain length of alcohol is increased, the window shifts towards lower alcohol fraction. Light scattering studies show that at very low lipid concentrations the tubules self-assemble directly from the isotropic phase where as for lipid concentrations greater than 4 mg/ml an intermediate L alpha phase is observed. These results indicate that the mechanism of tubule formation may be dependent on lipid concentration.

摘要

1,2-双(10,12-二十三碳二炔酰基)-sn-甘油-3-磷酸胆碱的水分散体在冷却至链熔化温度以下时，会形成被称为“微管”的中空圆柱形结构。我们研究了在甲醇/水、乙醇/水和正丙醇/水中微管的形成情况。对于每种醇，都存在一个特定的醇/水比例范围，在此范围内脂质会以微管形态沉淀。随着醇链长度的增加，该范围会向较低的醇分数方向移动。光散射研究表明，在非常低的脂质浓度下，微管直接从各向同性相自组装形成，而对于脂质浓度大于4 mg/ml的情况，则会观察到中间的Lα相。这些结果表明，微管形成的机制可能取决于脂质浓度。