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Sequence and in vitro translational analysis of a 1629-nucleotide ORF in Autographa californica nuclear polyhedrosis virus strain E2.

作者信息

Pham D Q, Sivasubramanian N

机构信息

Department of Entomology, University of California, Riverside 92521.

出版信息

Gene. 1992 Dec 15;122(2):345-8. doi: 10.1016/0378-1119(92)90224-d.

DOI:10.1016/0378-1119(92)90224-d
PMID:1487149
Abstract

The complete nucleotide (nt) sequence of an open reading frame (ORF) (map unit 5.1 to 3.8) from Autographa californica nuclear polyhedrosis virus strain E2 (AcMNPV-E2) has been determined. This 1629-nt ORF has a coding potential for a 61-kDa Pro-rich protein. However, in vitro translation of the 1629-nt ORF and sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE) revealed a 78-kDa protein product. The discrepancy between the M(r) predicted by the nt sequence and that obtained from the in vitro translational analysis is due to the high Pro content of this protein. The high Pro content causes anomalous migration of this protein during SDS-PAGE.

摘要

相似文献

1
Sequence and in vitro translational analysis of a 1629-nucleotide ORF in Autographa californica nuclear polyhedrosis virus strain E2.
Gene. 1992 Dec 15;122(2):345-8. doi: 10.1016/0378-1119(92)90224-d.
2
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Nucleotide sequence and transcript mapping of the HindIII F region of the Autographa californica nuclear polyhedrosis virus genome.苜蓿银纹夜蛾核型多角体病毒基因组HindIII F区的核苷酸序列及转录图谱
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The 1,629-nucleotide open reading frame located downstream of the Autographa californica nuclear polyhedrosis virus polyhedrin gene encodes a nucleocapsid-associated phosphoprotein.位于苜蓿银纹夜蛾核型多角体病毒多角体蛋白基因下游的1629个核苷酸的开放阅读框编码一种与核衣壳相关的磷蛋白。
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10
The pk-1 gene of Autographa californica multinucleocapsid nuclear polyhedrosis virus encodes a protein kinase.苜蓿银纹夜蛾多核衣壳核型多角体病毒的pk-1基因编码一种蛋白激酶。
J Gen Virol. 1994 Nov;75 ( Pt 11):2999-3006. doi: 10.1099/0022-1317-75-11-2999.

引用本文的文献

1
Molecular weight abnormalities of the CTCF transcription factor: CTCF migrates aberrantly in SDS-PAGE and the size of the expressed protein is affected by the UTRs and sequences within the coding region of the CTCF gene.CTCF转录因子的分子量异常：CTCF在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）中迁移异常，且所表达蛋白质的大小受CTCF基因编码区内非翻译区（UTRs）和序列的影响。
Nucleic Acids Res. 1997 Feb 1;25(3):466-74. doi: 10.1093/nar/25.3.466.
2
The 1,629-nucleotide open reading frame located downstream of the Autographa californica nuclear polyhedrosis virus polyhedrin gene encodes a nucleocapsid-associated phosphoprotein.位于苜蓿银纹夜蛾核型多角体病毒多角体蛋白基因下游的1629个核苷酸的开放阅读框编码一种与核衣壳相关的磷蛋白。
J Virol. 1993 Oct;67(10):5859-66. doi: 10.1128/JVI.67.10.5859-5866.1993.