Reilly L M, Guarino L A
Department of Entomology, Texas A&M University, College Station 77843.
J Gen Virol. 1994 Nov;75 ( Pt 11):2999-3006. doi: 10.1099/0022-1317-75-11-2999.
Open reading frame (ORF) 9 of the EcoRI I fragment of the baculovirus Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) genome encodes a protein, PK-1, which has strong similarity to serine-threonine protein kinases. The published sequence of the pk-1 gene contains an error that, when corrected, extends the ORF by 228 nucleotides. Transcription of pk-1 was first detectable by primer extension at 12 h post-infection, accumulating to high levels during the very late phase of infection. PK-1 produced in rabbit reticulocyte lysates was able to phosphorylate histone H1. Together, our results suggest that ORF 9 encodes a protein kinase that is expressed during the beginning of the late and throughout the very late phases of AcMNPV infection.
苜蓿银纹夜蛾多核衣壳核型多角体病毒（AcMNPV）基因组的EcoRI I片段的开放阅读框（ORF）9编码一种蛋白质PK-1，它与丝氨酸-苏氨酸蛋白激酶具有高度相似性。已发表的pk-1基因序列存在一个错误，校正后，开放阅读框延长了228个核苷酸。感染后12小时通过引物延伸首次检测到pk-1的转录，在感染的极晚期积累到高水平。在兔网织红细胞裂解物中产生的PK-1能够磷酸化组蛋白H1。我们的研究结果共同表明，开放阅读框9编码一种蛋白激酶，该激酶在AcMNPV感染的晚期开始阶段和整个极晚期表达。
