Robinson Alan S, Franz Gerald, Atkinson Peter W
Entomology Unit, FAO/IAEA Agriculture and Biotechnology Laboratory, Agency's Laboratories Seibersdorf, International Atomic Energy Agency, A-1400 Vienna, Austria.
Insect Biochem Mol Biol. 2004 Feb;34(2):113-20. doi: 10.1016/j.ibmb.2003.10.004.
The ability to genetically engineer insects other than Drosophila melanogaster has further extended modern genetic techniques into important insect pest species ranging from fruit fly pests of horticulture to mosquito vectors of human disease. In only a relatively short period of time, a range of transgenes have been inserted into more than 10 insect pest species. Genetic transformation of these pest species has proven to be a very important laboratory tool in analyzing gene function and effects on phenotype however the full extension of this technology into the field is yet to be realized. Here we briefly review the development of transgenic technology in pest insect species and discuss the challenges that remain in this applied area of insect genetics and entomology.
对黑腹果蝇以外的昆虫进行基因工程改造的能力，已将现代遗传技术进一步扩展到重要的害虫物种，从园艺果蝇害虫到人类疾病的蚊子传播媒介。在相对较短的时间内，一系列转基因已被插入到10多种害虫物种中。这些害虫物种的遗传转化已被证明是分析基因功能及其对表型影响的非常重要的实验室工具，然而，这项技术在田间的全面应用尚未实现。在此，我们简要回顾害虫昆虫物种中转基因技术的发展，并讨论昆虫遗传学和昆虫学这一应用领域中仍然存在的挑战。
