Pouliquen H, Keita D, Pinault L
Service de Pharmacie et Toxicologie, Ecole Nationale Vétérinaire de Nantes, France.
J Chromatogr. 1992 Dec 25;627(1-2):287-93. doi: 10.1016/0021-9673(92)87209-q.
A reversed-phase high-performance liquid chromatographic method was developed to detect oxytetracycline (OTC) in three species of marine shellfish (Crassostrea gigas, Ruditapes philippinarum and Scrobicularia plana). Shellfish tissues were analysed after solid-phase extraction by using a mobile phase containing acetonitrile and 0.02 M orthophosphoric acid solution. The linearity and precision of the method were checked over the concentration range 0.100-1.500 micrograms/g. The limits of detection and determination of OTC were 0.040 and 0.100 microgram/g, respectively. The recovery of OTC from spiked shellfish tissues was 79.8%. The OTC concentration in oyster (Crassostrea gigas) spiked at 0.500 microgram/g and stored at -20 degrees C was stable for 6 weeks. The method was applied to a 21-day experimental study performed with oysters.
