Pouliquen H, Gouelo D, Larhantec M, Pilet N, Pinault L
Ecole Nationale Vétérinaire de Nantes, Laboratoire de Chimiothérapie Aquacole et Environnement, Atlanpôle, La Chantrerie, France.
J Chromatogr B Biomed Sci Appl. 1997 Nov 21;702(1-2):157-62. doi: 10.1016/s0378-4347(97)00373-3.
A simple procedure for the determination of oxolinic acid (OA) and oxytetracycline (OTC), two antibacterial agents, in the shell of the blue mussel (Mytilus edulis), using reversed-phase high-performance liquid chromatography is described. Liquid chromatography was performed on a 5-microm LiChroSpher 100 RP-18E column using acetonitrile and a 0.02 M orthophosphoric acid solution as the mobile phase, with ultraviolet detection. After roughly grinding the shell, drugs were extracted using a methanolic oxalic acid solution. Linearity and precision were checked over the concentration range 0.04-0.32 microg/g. Limits of detection of OA and OTC were 0.012 and 0.008 microg/g, respectively. Mean extraction recoveries of OA and OTC from mussel shell were 72.9 and 65.4%, respectively. To demonstrate the usefulness of the analytical procedure, an experimental study was performed in blue mussels exposed to the drugs for eight days.
