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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Audsley N, McIntosh C, Phillips J E

Department of Zoology, University of British Columbia, Vancouver, Canada.

J Exp Biol. 1992 Dec;173:261-74. doi: 10.1242/jeb.173.1.261.

Schistocerca gregaria ion-transport peptide (Scg-ITP) was isolated from aqueous extracts of the corpus cardiacum by a four-step procedure, utilizing reverse-phase high-performance liquid chromatography for separation and stimulation of a Cl(-)-dependent short-circuit current (Isc) across locust ilea as the bioassay. 2. Scg-ITP has an unblocked N terminus and an apparent relative molecular mass of 7700. Thirty-one residues (of an estimated 65) were identified by sequence analysis. 3. Scg-ITP is structurally related to a crustacean family of neuropeptides which includes the crustacean hyperglycaemic hormones from the shore crab Carcinus maenas and the crayfish Orconectes limosus and moult-inhibiting hormone and vitellogenesis-inhibiting hormone from the lobster Homarus americanus. 4. Scg-ITP has no sequence homology with neuroparsins (Nps). Nps are the only other neuropeptides isolated to date that might regulate reabsorption in an insect hindgut (rectum).

从蝗虫心侧体的水提物中，通过四步操作分离出了沙漠蝗离子转运肽（Scg - ITP），利用反相高效液相色谱进行分离，并以刺激蝗虫回肠上依赖氯离子的短路电流（Isc）作为生物测定方法。2. Scg - ITP的N端未被封闭，表观相对分子质量为7700。通过序列分析鉴定出了（估计65个中的）31个残基。3. Scg - ITP在结构上与一类甲壳类神经肽家族相关，该家族包括来自海滨蟹平背蜞和小龙虾秀丽白虾的甲壳类高血糖激素，以及来自美洲龙虾的蜕皮抑制激素和卵黄发生抑制激素。4. Scg - ITP与神经促性腺素（Nps）没有序列同源性。Nps是迄今为止分离出的唯一可能调节昆虫后肠（直肠）重吸收的其他神经肽。

Audsley N, McIntosh C, Phillips J E

Department of Zoology, University of British Columbia, Vancouver, Canada.

J Exp Biol. 1992 Dec;173:261-74. doi: 10.1242/jeb.173.1.261.

Schistocerca gregaria ion-transport peptide (Scg-ITP) was isolated from aqueous extracts of the corpus cardiacum by a four-step procedure, utilizing reverse-phase high-performance liquid chromatography for separation and stimulation of a Cl(-)-dependent short-circuit current (Isc) across locust ilea as the bioassay. 2. Scg-ITP has an unblocked N terminus and an apparent relative molecular mass of 7700. Thirty-one residues (of an estimated 65) were identified by sequence analysis. 3. Scg-ITP is structurally related to a crustacean family of neuropeptides which includes the crustacean hyperglycaemic hormones from the shore crab Carcinus maenas and the crayfish Orconectes limosus and moult-inhibiting hormone and vitellogenesis-inhibiting hormone from the lobster Homarus americanus. 4. Scg-ITP has no sequence homology with neuroparsins (Nps). Nps are the only other neuropeptides isolated to date that might regulate reabsorption in an insect hindgut (rectum).

从蝗虫心侧体的水提物中，通过四步操作分离出了沙漠蝗离子转运肽（Scg - ITP），利用反相高效液相色谱进行分离，并以刺激蝗虫回肠上依赖氯离子的短路电流（Isc）作为生物测定方法。2. Scg - ITP的N端未被封闭，表观相对分子质量为7700。通过序列分析鉴定出了（估计65个中的）31个残基。3. Scg - ITP在结构上与一类甲壳类神经肽家族相关，该家族包括来自海滨蟹平背蜞和小龙虾秀丽白虾的甲壳类高血糖激素，以及来自美洲龙虾的蜕皮抑制激素和卵黄发生抑制激素。4. Scg - ITP与神经促性腺素（Nps）没有序列同源性。Nps是迄今为止分离出的唯一可能调节昆虫后肠（直肠）重吸收的其他神经肽。