使用高效液相色谱法测定血红蛋白A2

Haemoglobin A2 measurement using high performance liquid chromatography.

作者信息

Deacon-Smith R, Lord I

机构信息

Department of Haematology, Poole General Hospital, England, UK.

出版信息

Med Lab Sci. 1992 Jun;49(2):138-40.

PMID:1487979

Abstract

Haemoglobin A2 levels were assessed using a modular high performance liquid chromatography (HPLC) system with a protocol designed for the measurement of haemoglobin A1C. There was good correlation (r = 0.96; P < 0.001) between this technique and the International Committee for Standardisation in Haematology (ICSH) recommended method of microchromatography. The range of haemoglobin A2 was found to be 2.3-3.2% in apparently normal individuals, and 4.0-6.7% in those with beta-thalassaemia trait. The HPLC system produced reliable results quickly for haemoglobin A2 with no alteration to the protocol used for measuring haemoglobin A1C.

摘要

使用配备了专为测量糖化血红蛋白（HbA1C）设计的检测方案的模块化高效液相色谱（HPLC）系统评估血红蛋白A2（HbA2）水平。该技术与国际血液学标准化委员会（ICSH）推荐的微量色谱法之间具有良好的相关性（r = 0.96；P < 0.001）。在明显正常的个体中，HbA2范围为2.3 - 3.2%，在具有β地中海贫血特征的个体中为4.0 - 6.7%。HPLC系统能快速为HbA2产生可靠结果，且无需改变用于测量HbA1C的检测方案。