[First evaluation of the Variant haemoglobin analyzer (Bio-Rad)].

PURPOSE

The analytical performance of the first automated HPLC analyzer of haemoglobins A2 and Foetal is presented.

RESULTS

The analyzer shows an imprecision within-run between 0.66-2.7% and between-run 1.8-6.44. Correlation index with manual methods were r = 0.965 (p < 0.005) for Hb A2 and r = 0.999 (p < 0.0001) for Foetal Hb and the reference values of an adult population were established; Hb A2 (1.98-2.94%) and Hb F (0-0.95%). Several chromatograms of haemoglobinopathies are presented.

CONCLUSIONS

These results validate the method to resolve the increased demand of these parameters.