高效液相色谱法用于血红蛋白A2和F常规测定的评估。

Evaluation of high performance liquid chromatography for routine estimation of haemoglobins A2 and F.

作者信息

Tan G B, Aw T C, Dunstan R A, Lee S H

机构信息

Department of Laboratory Medicine, National University Hospital, Singapore.

出版信息

J Clin Pathol. 1993 Sep;46(9):852-6. doi: 10.1136/jcp.46.9.852.

DOI:10.1136/jcp.46.9.852

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC501524/

Abstract

AIMS

To compare high performance liquid chromatography (HPLC) with conventional methods for the estimation of blood haemoglobin A2 (HbA2) and haemoglobin F (HbF) concentrations in routine thalassemia screening.

METHODS

An HPLC system (the VARIANT Hemoglobin Testing System) was tested for precision and reproducibility in the measurement of HbA2 and HbF, and reference ranges were obtained for a local healthy adult population. HPLC was compared with column anion exchange chromatography for HbA2 measurement, and radial immunodiffusion, or alkaline denaturation for HbF measurement. The reliability of HbA2 measurement by HPLC for the detection of beta thalassaemia and HbE was assessed in 200 consecutive samples for routine thalassaemia screening.

RESULTS

HPLC was rapid, technically easy, and gave good precision and reproducibility. In all comparisons linear regression analysis showed good correlation between HbA2 or HbF concentrations determined by HPLC and by the respective conventional methods. All beta thalassaemia or haemoglobin E carriers presumptively identified by conventional methods in 200 consecutive samples were detected by HbA2 measurement using HPLC, without any false positive or false negative results.

CONCLUSIONS

The measurement of HbA2 and HbF by HPLC is rapid, reproducible, and precise. It is as reliable as column chromatography for the measurement of HbA2 and radial immunodiffusion or alkaline denaturation for the measurement of HbF. HPLC may be an appropriate method for rapid screening in population surveys for beta thalassaemia and HbE carriers.

摘要

目的

在常规地中海贫血筛查中，比较高效液相色谱法（HPLC）与传统方法用于估算血液中血红蛋白A2（HbA2）和血红蛋白F（HbF）浓度的效果。

方法

对一种HPLC系统（VARIANT血红蛋白检测系统）进行了HbA2和HbF测量的精密度和重现性测试，并获得了当地健康成年人群的参考范围。将HPLC与用于HbA2测量的柱阴离子交换色谱法，以及用于HbF测量的放射免疫扩散法或碱性变性法进行了比较。在200份用于常规地中海贫血筛查的连续样本中，评估了HPLC测量HbA2用于检测β地中海贫血和HbE的可靠性。

结果

HPLC快速、技术操作简便，具有良好的精密度和重现性。在所有比较中，线性回归分析表明，HPLC测定的HbA2或HbF浓度与相应传统方法测定的结果之间具有良好的相关性。在200份连续样本中，所有通过传统方法推定鉴定出的β地中海贫血或血红蛋白E携带者，使用HPLC测量HbA2均能检测出来，无任何假阳性或假阴性结果。

结论

用HPLC测量HbA2和HbF快速、可重现且精确。在测量HbA2方面，它与柱色谱法一样可靠；在测量HbF方面，它与放射免疫扩散法或碱性变性法一样可靠。HPLC可能是在人群调查中快速筛查β地中海贫血和HbE携带者的合适方法。