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使用商用乳胶凝集试剂盒进行嗜肺军团菌菌种鉴定:与血清型12存在潜在交叉反应问题。

Legionella pneumophila species identification using a commercial latex agglutination kit: a potential cross-reaction problem with serogroup 12.

作者信息

Richardson I R, Lightfoot N F

机构信息

Newcastle Public Health Laboratory, Institute of Pathology, General Hospital, Newcastle upon Tyne, England, UK.

出版信息

Med Lab Sci. 1992 Jun;49(2):144-6.

PMID:1487981
Abstract

Seventy-nine strains of legionella spp. and 13 non-legionella isolates were tested using a commercial latex agglutination kit. Cross-reactions were observed for all strains of Legionella pneumophila serogroup 12 examined between latex particles coated with rabbit antibodies raised against L. pneumophila serogroup 1 and those coated with rabbit antibodies to serogroups 2-14. Strains of L. pneumophila serogroup 1 did not cross-react with latex particles directed against L. pneumophila serogroups 2-14. This suggests that L. pneumophila serogroup 12 shares common surface antigens with L. pneumophila serogroup 1, and that conversely serogroup 1 isolates do not share major surface antigen determinants with serogroups 2-14. All other isolates tested gave expected results.

摘要

使用一种商用乳胶凝集试剂盒对79株军团菌属菌株和13株非军团菌分离株进行了检测。在针对嗜肺军团菌血清群1制备的兔抗体包被的乳胶颗粒与针对血清群2 - 14的兔抗体包被的乳胶颗粒之间,观察到所有检测的嗜肺军团菌血清群12菌株均出现交叉反应。嗜肺军团菌血清群1的菌株与针对嗜肺军团菌血清群2 - 14的乳胶颗粒未发生交叉反应。这表明嗜肺军团菌血清群12与嗜肺军团菌血清群1具有共同的表面抗原,相反,血清群1的分离株与血清群2 - 14不共享主要的表面抗原决定簇。所有其他检测的分离株均给出了预期结果。

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J Clin Microbiol. 1994 Dec;32(12):3054-5. doi: 10.1128/jcm.32.12.3054-3055.1994.