评估Duopath军团菌侧向流动分析法用于鉴定嗜肺军团菌和军团菌属培养分离株。
Evaluation of the Duopath Legionella lateral flow assay for identification of Legionella pneumophila and Legionella species culture isolates.
作者信息
Helbig Jürgen Herbert, Lück Paul Christian, Kunz Britta, Bubert Andreas
机构信息
Institute of Medical Microbiology and Hygiene, Medical Faculty of the Technical University Dresden, Fetscherstr. 74, D-01307 Dresden, Germany.
出版信息
Appl Environ Microbiol. 2006 Jun;72(6):4489-91. doi: 10.1128/AEM.00346-06.
Duopath Legionella (Merck KGaA, Darmstadt, Germany) is a new immunochromatographic assay for the simultaneous identification of cultured L. pneumophila and Legionella species other than L. pneumophila. In tests of 89 L. pneumophila strains and 87 Legionella strains other than L. pneumophila representing 41 different species, Duopath and a widely used latex agglutination assay detected L. pneumophila with 100% and 98% accuracy, respectively, whereas the percentages differed significantly for other Legionella spp. (93% versus 37% [P < 0.001]). Since many countries' regulations require the identification of Legionella spp. in water and environmental samples, the use of Duopath Legionella to comply with those regulations could contribute to significantly fewer false-negative results.
杜奥帕特嗜肺军团菌检测试剂（德国默克集团，达姆施塔特）是一种新型免疫层析检测法，可同时鉴定培养的嗜肺军团菌和除嗜肺军团菌之外的其他军团菌属菌种。在对代表41个不同菌种的89株嗜肺军团菌菌株和87株非嗜肺军团菌的军团菌菌株进行检测时，杜奥帕特试剂和一种广泛使用的乳胶凝集检测法检测嗜肺军团菌的准确率分别为100%和98%，而对于其他军团菌属菌种，二者的检测百分比差异显著（93%对37% [P < 0.001]）。由于许多国家的法规要求对水和环境样本中的军团菌属菌种进行鉴定，使用杜奥帕特嗜肺军团菌检测试剂来遵守这些法规可能会显著减少假阴性结果。
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