Salvetti M, Buttinelli C, Ristori G, Carbonari M, Cherchi M, Fiorelli M, Grasso M G, Toma L, Pozzilli C
Department of Neurological Sciences, Università La Sapienza, Rome, Italy.
J Autoimmun. 1992 Dec;5(6):691-702. doi: 10.1016/0896-8411(92)90186-t.
Owing to their conservation and immunogenicity, heat shock proteins (hsps) represent a class of potential autoantigens. Moreover, they could be targets for gamma delta T lymphocytes, which are prominent in various immune disorders. We studied the T cell proliferative primary responses to recombinant M. bovis 65 kDa hsp (hsp65) and M. tuberculosis 70 kDa hsp (hsp70) in 31 patients with multiple sclerosis (MS), 19 patients with other neurological diseases (OND) and 19 healthy individuals. Positive responses to hsp70, but not to hsp65 were significantly more frequent in patients with MS than in patients with OND or in healthy individuals. In order to verify and refine these results and to characterize the hsp reactive T lymphocytes, we screened 147 PPD-specific long-term T cell lines (76 from 10 patients with MS and 71 from 12 healthy donors) for their proliferative response to hsp65 and hsp70. hsp70-reactive T lines were significantly more common in patients with MS than in healthy controls. The number of T lines responding to hsp65 increased in the MS group only slightly. In 19 T lymphocyte lines from patients with MS and healthy donors, a cytofluorometric analysis was performed with special attention paid to distinct T cell receptor gamma delta determinants. With one exception, in each line the population of gamma delta T cells remained a minority. We conclude that an increased T cell response to mycobacterial hsp70 may be present in patients with multiple sclerosis.
由于其保守性和免疫原性，热休克蛋白（hsps）是一类潜在的自身抗原。此外，它们可能是γδT淋巴细胞的靶标，γδT淋巴细胞在各种免疫紊乱中很突出。我们研究了31例多发性硬化症（MS）患者、19例其他神经系统疾病（OND）患者和19名健康个体对重组牛分枝杆菌65 kDa热休克蛋白（hsp65）和结核分枝杆菌70 kDa热休克蛋白（hsp70）的T细胞增殖初级反应。MS患者对hsp70而非hsp65的阳性反应明显比OND患者或健康个体更频繁。为了验证和完善这些结果并表征hsp反应性T淋巴细胞，我们筛选了147个PPD特异性长期T细胞系（76个来自10例MS患者，71个来自12名健康供体）对hsp65和hsp70的增殖反应。hsp70反应性T细胞系在MS患者中比健康对照明显更常见。MS组中对hsp65有反应的T细胞系数量仅略有增加。对19个来自MS患者和健康供体的T淋巴细胞系进行了细胞荧光分析，特别关注不同的T细胞受体γδ决定簇。除一个例外，在每个细胞系中γδT细胞群体仍然是少数。我们得出结论，多发性硬化症患者可能存在对分枝杆菌hsp70的T细胞反应增加。
