用分枝杆菌的分枝菌酸阿拉伯半乳聚糖肽聚糖刺激后牛γδ T细胞产生γ干扰素。
Gamma interferon production by bovine gamma delta T cells following stimulation with mycobacterial mycolylarabinogalactan peptidoglycan.
作者信息
Vesosky B, Turner O C, Turner J, Orme I M
机构信息
Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, 80523, USA.
出版信息
Infect Immun. 2004 Aug;72(8):4612-8. doi: 10.1128/IAI.72.8.4612-4618.2004.
A large percentage of lymphocytes in the blood of cattle express the gamma delta T-cell receptor, but specific functions for these cells have not yet been clearly defined. There is evidence, however, that human, murine, and bovine gamma delta T cells have a role in the immune response to mycobacteria. This study investigated the ability of bovine gamma delta T cells to expand and produce gamma interferon (IFN-gamma) in response to stimulation with mycobacterial products. Bovine gamma delta T cells, isolated from the peripheral blood of healthy cattle, expanded following in vitro stimulation with live mycobacteria, mycobacterial crude cell wall extract, and Mycobacterium bovis culture filtrate proteins. In addition, purified gamma delta T cells, cocultured with purified monocytes and interleukin-2, consistently produced significant amounts of IFN-gamma in response to mycobacterial cell wall. The IFN-gamma-inducing component of the cell wall was further identified as a proteolytically resistant, non-sodium dodecyl sulfate-soluble component of the mycolylarabinogalactan peptidoglycan.
牛血液中很大比例的淋巴细胞表达γδ T细胞受体，但这些细胞的具体功能尚未明确界定。然而，有证据表明，人类、小鼠和牛的γδ T细胞在对分枝杆菌的免疫反应中发挥作用。本研究调查了牛γδ T细胞在受到分枝杆菌产物刺激后扩增并产生γ干扰素（IFN-γ）的能力。从健康牛外周血中分离出的牛γδ T细胞，在受到活分枝杆菌、分枝杆菌粗细胞壁提取物和牛分枝杆菌培养滤液蛋白的体外刺激后会扩增。此外，纯化的γδ T细胞与纯化的单核细胞和白细胞介素-2共培养时，在受到分枝杆菌细胞壁刺激后会持续产生大量的IFN-γ。细胞壁的IFN-γ诱导成分进一步被鉴定为霉菌阿拉伯半乳聚糖肽聚糖的一种抗蛋白水解、不溶于十二烷基硫酸钠的成分。
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