Gao Z R, Hao Z Q, Li Y, Im M J, Spence R J
Department of Burn Surgery, Affiliated Hospital of Qinghai Medical College, Xining, People's Republic of China.
Burns. 1992 Dec;18(6):492-6. doi: 10.1016/0305-4179(92)90183-u.
Collagen was extracted by pepsin digestion from porcine skin, and collagen membrane was prepared by salt precipitation. The porcine collagen membrane was evaluated as a burn wound dressing in deep partial skin thickness burn wounds in rats. Burn wounds, 4 x 4 cm, were inflicted by exposure of skin to 75 degrees C for 15 s followed by de-epithelialization. Wound healing was assessed by planimetry of epithelialization on day 10 after injury. Open wounds exhibited 24 per cent of wound area re-epithelialized. Collagen membrane dressing significantly improved the healing to 69 per cent of wound area (P < 0.0001). In a completely separate experiment, the porcine collagen membrane was applied as a wound dressing to the donor sites of burn patients, and its effect on wound healing was compared with that of a petroleum jelly gauze dressing. The donor sites covered with petroleum jelly gauze had re-epithelialized by an average of 14.5 days (ranging from 13 to 16 days) after wounding. The wounds dressed with collagen membrane demonstrated a significant increase in the healing rate. Complete re-epithelialization was observed by 10.3 days (ranging from 10 to 12 days) after wounding (P < 0.0001).
通过胃蛋白酶消化从猪皮中提取胶原蛋白，并通过盐析制备胶原膜。将猪胶原膜作为大鼠深Ⅱ度烧伤创面的烧伤创面敷料进行评估。通过将皮肤暴露于75摄氏度15秒然后去除上皮来造成4×4厘米的烧伤创面。在受伤后第10天通过上皮化面积测量法评估伤口愈合情况。开放性伤口显示24%的伤口面积重新上皮化。胶原膜敷料显著改善愈合情况，使伤口面积的愈合率达到69%（P<0.0001）。在一个完全独立的实验中，将猪胶原膜作为伤口敷料应用于烧伤患者的供皮区，并将其对伤口愈合的效果与凡士林纱布敷料进行比较。覆盖凡士林纱布的供皮区在受伤后平均14.5天（范围为13至16天）重新上皮化。用胶原膜包扎的伤口愈合速度显著加快。受伤后10.3天（范围为10至12天）观察到完全重新上皮化（P<0.0001）。
