Sarría L, Gallego L, Lardelli P, Manzano D, Basaras M, Cisterna R
Departamento de Microbiología e Inmunología, Facultad de Medicina y Odontología, Universidad del País Vasco.
Enferm Infecc Microbiol Clin. 1992 Oct;10(8):470-3.
The presence of hepatitis delta virus was investigated in liver biopsies by in situ cyto-hybridization with a probe labelled with digoxigenin.
The techniques developed included extraction of plasmid DNA by lysis by alkali, electroelution, electrophoresis in agarose gel and digoxigenin labelling, and the application in liver tissue.
Viral RNA was detected in 6 of the 10 patients, and the reactivity was exclusively restricted to the nucleolus of the hepatocytes.
This method reveals as a sensitive and quick diagnostic procedure, which allows to study the intensity of the infection as well as the serological state of the patient.
采用地高辛标记探针原位细胞杂交技术，对肝活检组织中的丁型肝炎病毒进行检测。
所采用的技术包括碱裂解法提取质粒DNA、电洗脱、琼脂糖凝胶电泳及地高辛标记，以及在肝组织中的应用。
10例患者中有6例检测到病毒RNA，且反应仅局限于肝细胞的核仁。
该方法是一种敏感、快速的诊断方法，可用于研究感染强度及患者的血清学状态。
