Sorochinskiĭ V V, Kurganov B I
Biokhimiia. 1992 Nov;57(11):1603-10.
A theoretical analysis of the functioning of membrane biosensors based on consecutive polyenzymatic transformations of the substrate and detectable by indicator electrodes with electrochemical regeneration of the enzyme active sites, has been carried out. Correlations between the substrate concentration, diffusion-catalytic characteristics of the coating components, and rate of the indicator reaction on the one hand, and the level of the steady-state response of the polyenzymatic biosensors, on the other, have been determined for relatively low values of the polyenzymatic layer thickness. Various regimens of functioning of bienzymatic electrodes have been considered.
对基于底物连续多酶转化且可通过酶活性位点电化学再生的指示电极进行检测的膜生物传感器的功能进行了理论分析。对于相对较低的多酶层厚度值，已确定了底物浓度、涂层成分的扩散催化特性和指示反应速率与多酶生物传感器稳态响应水平之间的相关性。考虑了双酶电极的各种功能模式。
