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一种抗冻多肽与冰结合的模型。

A model for binding of an antifreeze polypeptide to ice.

作者信息

Wen D, Laursen R A

机构信息

Department of Chemistry, Boston University, Massachusetts 02215.

出版信息

Biophys J. 1992 Dec;63(6):1659-62. doi: 10.1016/S0006-3495(92)81750-2.

DOI:10.1016/S0006-3495(92)81750-2
PMID:1489916
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1262283/
Abstract

A model is proposed, based on recent peptide analog and ice crystal etching studies, whereby an alanine-rich, alpha-helical antifreeze polypeptide (AFP) from the winter flounder inhibits the growth of ice crystals by hydrogen bonding of Thr, Asn, and Asp side chains in a specific pattern to the [2021] hexagonal bipyramidal planes of ice. It is further suggested that this mode of binding is unidirectional, maximizing opportunities for packing of AFPs on the ice surface, and that ice crystal growth inhibition occurs by a two-step mechanism involving hydrogen bonding and hydrophobic interpeptide interactions.

摘要

基于最近的肽类似物和冰晶蚀刻研究，提出了一个模型，据此，来自冬季比目鱼的富含丙氨酸的α-螺旋抗冻多肽（AFP）通过苏氨酸、天冬酰胺和天冬氨酸侧链以特定模式与冰的[2021]六方双锥面形成氢键来抑制冰晶生长。还进一步表明，这种结合模式是单向的，使AFP在冰表面堆积的机会最大化，并且冰晶生长抑制是通过涉及氢键和疏水肽间相互作用的两步机制发生的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55e4/1262283/90abf0a64de0/biophysj00094-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55e4/1262283/90abf0a64de0/biophysj00094-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55e4/1262283/90abf0a64de0/biophysj00094-0210-a.jpg

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1
A model for binding of an antifreeze polypeptide to ice.一种抗冻多肽与冰结合的模型。
Biophys J. 1992 Dec;63(6):1659-62. doi: 10.1016/S0006-3495(92)81750-2.
2
Ice-binding mechanism of winter flounder antifreeze proteins.冬鲽抗冻蛋白的冰结合机制。
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Mechanisms of antifreeze proteins investigated via the site-directed spin labeling technique.通过定点自旋标记技术研究抗冻蛋白的机制。
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Balance between hydration enthalpy and entropy is important for ice binding surfaces in Antifreeze Proteins.

本文引用的文献

1
Antifreeze glycoproteins from polar fish blood.来自极地鱼类血液的抗冻糖蛋白。
Annu Rev Biophys Biophys Chem. 1986;15:59-78. doi: 10.1146/annurev.bb.15.060186.000423.
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Crystal structure of an antifreeze polypeptide and its mechanistic implications.一种抗冻多肽的晶体结构及其机制意义
Nature. 1988 May 19;333(6170):232-7. doi: 10.1038/333232a0.
3
Biochemistry of fish antifreeze proteins.鱼类抗冻蛋白的生物化学
对于抗冻蛋白中的冰结合表面而言，水合焓与熵之间的平衡很重要。
Sci Rep. 2017 Sep 19;7(1):11901. doi: 10.1038/s41598-017-11982-8.
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Sci Rep. 2017 Feb 13;7:42501. doi: 10.1038/srep42501.
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Antifreeze glycopeptides: from structure and activity studies to current approaches in chemical synthesis.抗冻糖肽：从结构与活性研究到化学合成的当前方法
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The biological function of an insect antifreeze protein simulated by molecular dynamics.通过分子动力学模拟昆虫抗冻蛋白的生物学功能。
Elife. 2015 May 7;4:e05142. doi: 10.7554/eLife.05142.
7
Effects of three different types of antifreeze proteins on mouse ovarian tissue cryopreservation and transplantation.三种不同类型的抗冻蛋白对小鼠卵巢组织冷冻保存及移植的影响。
PLoS One. 2015 May 4;10(5):e0126252. doi: 10.1371/journal.pone.0126252. eCollection 2015.
8
Antifreeze protein-induced selective crystallization of a new thermodynamically and kinetically less preferred molecular crystal.抗冻蛋白诱导新的热力学和动力学上较不优先的分子晶体的选择性结晶。
Chemistry. 2013 Nov 18;19(47):16104-12. doi: 10.1002/chem.201302049. Epub 2013 Oct 9.
9
Thermodynamic Analysis of Thermal Hysteresis: Mechanistic Insights into Biological Antifreezes.热滞的热力学分析：对生物防冻剂的机理洞察
J Chem Thermodyn. 2012 Oct 1;53:125-130. doi: 10.1016/j.jct.2012.04.028. Epub 2012 May 7.
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Structure and interactions of fish type III antifreeze protein in solution.鱼型 III 抗冻蛋白在溶液中的结构和相互作用。
Biophys J. 2010 Jul 21;99(2):609-18. doi: 10.1016/j.bpj.2010.04.030.
FASEB J. 1990 May;4(8):2460-8. doi: 10.1096/fasebj.4.8.2185972.
4
Adsorption of alpha-helical antifreeze peptides on specific ice crystal surface planes.α-螺旋抗冻肽在特定冰晶表面平面上的吸附
Biophys J. 1991 Feb;59(2):409-18. doi: 10.1016/S0006-3495(91)82234-2.
5
Energy-optimized structure of antifreeze protein and its binding mechanism.抗冻蛋白的能量优化结构及其结合机制。
J Mol Biol. 1992 Jan 20;223(2):509-17. doi: 10.1016/0022-2836(92)90666-8.
6
Structure-function relationships in an antifreeze polypeptide. The role of neutral, polar amino acids.一种抗冻多肽中的结构-功能关系。中性、极性氨基酸的作用。
J Biol Chem. 1992 Jul 15;267(20):14102-8.
7
Adsorption inhibition as a mechanism of freezing resistance in polar fishes.吸附抑制作为极地鱼类抗冻机制
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2589-93. doi: 10.1073/pnas.74.6.2589.