TAMM I, HORSFALL F L
J Exp Med. 1952 Jan;95(1):71-97. doi: 10.1084/jem.95.1.71.
A mucoprotein, present in normal human urine, has been isolated and obtained in a state of a high degree of purity. A number of the biological, chemical, and physicochemical properties of the substance have been studied. From the results obtained in the present investigation and those reported in succeeding papers (34, 35) it appears that the mucoprotein has a high molecular weight, i.e., of the order of 7.0 x 10(6), consists of thread-like molecules which have axial ratios of approximately 100, and is specifically antigenic. This substance, which appears to be free of contaminating material, possesses in extraordinary degree the capacity to react with influenza, mumps, and Newcastle disease viruses. At equilibrium, with influenza virus, the minimal amount of the substance capable of giving a demonstrable reaction with one hemagglutinating unit of virus appears to be about 0.0003 microg. The mucoprotein is altered by preparations of influenza viruses and its capacity to react with these agents or others is lost. The kinetics of the inactivation process brought about by influenza viruses is in accord with those of well known enzyme-substrate systems. With the exception of the capacity to react with viruses, altered mucoprotein did not differ from the native substance relative to any of the properties examined in the present study. That certain physicochemical properties of the altered mucoprotein are different from those of the native substance is demonstrated in succeeding papers (34, 35).
一种存在于正常人尿液中的粘蛋白已被分离出来，并获得了高纯度状态。已对该物质的一些生物学、化学和物理化学性质进行了研究。从本研究获得的结果以及后续论文（34、35）中报道的结果来看，这种粘蛋白具有高分子量，即约为7.0×10⁶，由轴比约为100的线状分子组成，并且具有特异性抗原性。这种似乎不含污染物的物质具有与流感、腮腺炎和新城疫病毒发生反应的非凡能力。在与流感病毒达到平衡时，与一个血凝单位病毒能产生可证实反应的该物质的最小量似乎约为0.0003微克。粘蛋白会被流感病毒制剂改变，并且其与这些病原体或其他病原体发生反应的能力会丧失。由流感病毒引起的失活过程的动力学与众所周知的酶 - 底物系统的动力学一致。除了与病毒发生反应的能力外，相对于本研究中检测的任何性质，改变后的粘蛋白与天然物质没有差异。改变后的粘蛋白的某些物理化学性质与天然物质不同这一点在后续论文（34、35）中得到了证明。
