在黄嘌呤存在的情况下，采用反相高效液相色谱法测定人尿液样本中的乙酰唑胺。
Determination of acetazolamide in human urine samples by reversed-phase high-performance liquid chromatography in the presence of xanthines.
作者信息
Herráez-Hernández R, Campíns-Falcó P, Sevillano-Cabeza A
机构信息
Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot, Spain.
出版信息
J Chromatogr. 1992 Nov 6;582(1-2):181-7. doi: 10.1016/0378-4347(92)80317-j.
A simple, rapid and selective high-performance liquid chromatographic assay for the determination of acetazolamide in urine samples is described. After extraction with ethyl acetate, the drug is chromatographed on an HP-Hypersil ODS-C18 column with a mobile phase of acetonitrile-phosphate buffer (pH 3) and ultraviolet detection at 275 nm. The efficiency of the extraction, the linearity and the reproducibility of the method permit the evaluation of acetazolamide urinary excretion a long time after its administration.
本文描述了一种用于测定尿样中乙酰唑胺的简单、快速且选择性高的高效液相色谱法。用乙酸乙酯萃取后，将药物在HP-Hypersil ODS-C18柱上进行色谱分析，流动相为乙腈-磷酸盐缓冲液（pH 3），并在275 nm处进行紫外检测。该方法的萃取效率、线性和重现性允许在给药后很长时间评估乙酰唑胺的尿排泄情况。
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