Law S W, Conneely O M, DeMayo F J, O'Malley B W
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Mol Endocrinol. 1992 Dec;6(12):2129-35. doi: 10.1210/mend.6.12.1491694.
We have identified and cloned a novel member of the nuclear receptor superfamily. The cDNA was isolated from a mouse brain cDNA library and encodes a protein 598 amino acids in length with a predicted mol wt of 66 kilodaltons. The amino acid sequence of the protein is closely related to an additional family member and immediate early gene product, Nur77, and the novel factor is referred to as Nurr1 (Nur-related factor 1). The relationship between Nurr1 and Nur77 suggests that these proteins constitute an additional subfamily within the nuclear receptor superfamily. Like Nur77, the expression of Nurr1 is induced by membrane depolarization of PC12 cells. However, while Nur77 shows an early transcriptional response to nerve growth factor stimulation, the failure of Nurr1 to respond to this agent suggests a differential selectivity of the two proteins in terms of their transcriptional responses to specific stimuli. Finally, both proteins are differentially expressed during development and in tissues of the adult mouse. Unlike Nur77, Nurr1 appears to be predominantly located in brain tissue, suggesting a primary role for this putative transcription factor in regulation of gene expression in the central nervous system.
我们已鉴定并克隆了核受体超家族的一个新成员。该cDNA是从小鼠脑cDNA文库中分离得到的，编码一个长度为598个氨基酸的蛋白质，预测分子量为66千道尔顿。该蛋白质的氨基酸序列与另一个家族成员及早期即刻基因产物Nur77密切相关，这个新因子被称为Nurr1（Nur相关因子1）。Nurr1与Nur77之间的关系表明，这些蛋白质构成了核受体超家族中的一个新亚家族。与Nur77一样，Nurr1的表达可被PC12细胞的膜去极化诱导。然而，虽然Nur77对神经生长因子刺激表现出早期转录反应，但Nurr1对该因子无反应，这表明这两种蛋白质在对特定刺激的转录反应方面具有不同的选择性。最后，这两种蛋白质在成年小鼠的发育过程中和组织中表达存在差异。与Nur77不同，Nurr1似乎主要位于脑组织中，这表明这个假定的转录因子在中枢神经系统基因表达调控中起主要作用。
