Fujita T, Itakura M
J Lab Clin Med. 1978 Jul;92(1):135-40.
The relationship between lysozyme and sodium reabsorption by the kidney tubule was studied in the experimental Fanconi syndrome. Female, anesthetized Sprague-Dawley rats were injected intravenously with maleic acid (an inhibitor of sodium transport) neutralized with sodium hydroxide in doses of either 2 or 8 mmol/kg. Clearance studies were performed immediately afterward, and plasma and urine were analyzed for inulin, pH, sodium, glucose, and lysozyme. Two hours after the maleic acid injection, renal cortical tissue was removed and homogenized. Specific activity of Na-K-ATPase was assayed in the light microsomal fraction. The results showed that both concentrations of maleic acid caused significant increases in urinary volume, glucose excretion, and pH. There were significantly correlated decreases in TNafract and TLyfract. The slope of the regression line (TLyfract = 1.03 TNafract - 5.82; r = 0.92) approximated unity. Renal cortical Na-K-ATPase activity was significantly decreased by 25% in the animals receiving 2 mmol maleic acid and 43% in the animals receiving 8 mmol. The evidence suggests that lysozyme reabsorption in the proximal tubule might be mediated directly or indirectly by active tubular transport of sodium, a process that is related to the Na-K-ATPase transport system.
在实验性范科尼综合征中研究了溶菌酶与肾小管钠重吸收之间的关系。对雌性、麻醉的斯普拉格 - 道利大鼠静脉注射用氢氧化钠中和的马来酸（钠转运抑制剂），剂量为2或8 mmol/kg。随后立即进行清除率研究，并分析血浆和尿液中的菊粉、pH值、钠、葡萄糖和溶菌酶。注射马来酸两小时后，取出肾皮质组织并匀浆。在轻微粒体部分测定Na-K-ATP酶的比活性。结果表明，两种浓度的马来酸均导致尿量、葡萄糖排泄和pH值显著增加。TNafract和TLyfract显著相关降低。回归线斜率（TLyfract = 1.03 TNafract - 5.82；r = 0.92）接近1。接受2 mmol马来酸的动物肾皮质Na-K-ATP酶活性显著降低25%，接受8 mmol的动物降低43%。证据表明，近端小管中的溶菌酶重吸收可能直接或间接由钠的主动肾小管转运介导，这一过程与Na-K-ATP酶转运系统有关。
