通过体内脑微透析研究局部氰化物灌注对大鼠纹状体细胞外多巴胺及其代谢产物的影响。

Effect of local cyanide perfusion on rat striatal extracellular dopamine and its metabolites as studied by in vivo brain microdialysis.

作者信息

Kiuchi Y, Inagaki M, Izumi J, Matsumoto M, Yamazaki Y, Oguchi K

机构信息

Department of Pharmacology, School of Medicine, Showa University, Tokyo, Japan.

出版信息

Neurosci Lett. 1992 Dec 7;147(2):193-6. doi: 10.1016/0304-3940(92)90593-v.

DOI:10.1016/0304-3940(92)90593-v

PMID:1491807

Abstract

To investigate in vivo effects of energy failure on functions of dopaminergic neurons, we administered 0 (control), 0.2, 1 or 2 mM sodium cyanide (NaCN) dissolved in Ringer's solution for 60 min into the rat striatum through a brain microdialysis membrane. During NaCN perfusion, a transient and concentration-dependent increase in dopamine (DA) levels in the dialysate was observed. The maximum DA level during 2 mM NaCN perfusion was found to be 63-fold higher than the control levels. Leves of 3,4-dihydroxyphenylacetic acid and homovanillic acid were continuously lowered during and after NaCN perfusion. These data suggest that suppression of ATP production by NaCN induces an abrupt and remarkable increase in dopamine release from the nerve terminal in the striatum.

摘要

为研究能量衰竭对多巴胺能神经元功能的体内影响，我们通过脑微透析膜向大鼠纹状体内注射溶解于林格氏液中的0（对照）、0.2、1或2 mM氰化钠（NaCN），持续60分钟。在NaCN灌注期间，观察到透析液中多巴胺（DA）水平出现短暂且浓度依赖性的升高。发现在2 mM NaCN灌注期间的最大DA水平比对照水平高63倍。在NaCN灌注期间及之后，3,4-二羟基苯乙酸和高香草酸水平持续降低。这些数据表明，NaCN抑制ATP生成会导致纹状体神经末梢多巴胺释放突然且显著增加。