Peinado P, Martínez-Liarte J H, Solano F, Lozano J A
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Murcia, Spain.
Pigment Cell Res. 1992 Dec;5(6):400-3. doi: 10.1111/j.1600-0749.1992.tb00570.x.
The antifungal reagent Fungizone (amphotericin B and deoxycholate) caused an activation in dopachrome tautomerase and dopa oxidase activities of B16/F10 melanoma cells at the routine concentration (2.5 micrograms/ml) used for preventing molds and yeast growth in cultures of animal cells. However, higher amphotericin B concentrations caused a significant cell death and the inhibition of enzymatic activities. At the optimal concentration of Fungizone, the enzymatic activities and melanin content were augmented as incubation time increased. The detergent sodium deoxycholate alone exerted no effect on these melanogenic parameters, eliminating the possibility that this detergent was partially responsible for melanogenic modifications produced by Fungizone. After withdrawal of Fungizone from the reaction medium, the recovery of melanogenic parameters to normal values was slower for DCT than for tyrosinase. The behavior of dopa oxidase was very similar to that reported by Johnson and Bagnara (Pigment Cell Res. 3, 173-175) for tyrosine hydroxylase.
抗真菌试剂两性霉素B（两性霉素B与脱氧胆酸盐）在用于防止动物细胞培养物中霉菌和酵母菌生长的常规浓度（2.5微克/毫升）下，可激活B16/F10黑色素瘤细胞的多巴色素互变异构酶和多巴氧化酶活性。然而，更高浓度的两性霉素B会导致显著的细胞死亡并抑制酶活性。在两性霉素B的最佳浓度下，随着孵育时间的增加，酶活性和黑色素含量会增加。单独的去污剂脱氧胆酸钠对这些黑色素生成参数没有影响，排除了这种去污剂部分导致两性霉素B产生黑色素生成修饰的可能性。从反应介质中去除两性霉素B后，多巴色素互变异构酶（DCT）的黑色素生成参数恢复到正常值的速度比酪氨酸酶慢。多巴氧化酶的行为与约翰逊和巴尼亚拉（《色素细胞研究》3，173 - 175）报道的酪氨酸羟化酶的行为非常相似。
