多种生长因子对软骨细胞分化模型的影响。

Effects of various growth factors on a chondrocyte differentiation model.

作者信息

Suzuki F

机构信息

Department of Biochemistry, Faculty of Dentistry, Osaka University, Japan.

出版信息

Adv Exp Med Biol. 1992;324:101-6. doi: 10.1007/978-1-4615-3398-6_10.

DOI:10.1007/978-1-4615-3398-6_10

PMID:1492610

Abstract

We developed a new 4-dimensional differentiation model of growth-cartilage cells (temporally and spatially regulated). composed of a high-density suspension culture of rabbit costal chondrocytes. In this system, fibroblast growth factor (FGF) or transforming growth factor-beta (TGF beta) stimulated proliferation and matrix synthesis, but reversibly inhibited terminal differentiation-induction of alkaline phosphatase (Alpase) activity, type X collagen synthesis and subsequent calcification. When parathyroid hormone (PTH) was added to the differentiation model, both the induction of Alpase and calcium uptake were reversibly suppressed. In contrast, calcitonin (CT) stimulated Alpase activity and calcium uptake dose-dependently. Bone morphogenetic protein (BMP) strongly stimulated DNA synthesis of chondrocytes in the presence of FGF and induced rapid maturation of chondrocytes at a growing stage. Moreover, BMP stimulated Alpase activity in multilayered chondrocytes at the calcifying stage.

摘要

我们开发了一种新的生长软骨细胞的四维分化模型（时空调节），该模型由兔肋软骨细胞的高密度悬浮培养组成。在这个系统中，成纤维细胞生长因子（FGF）或转化生长因子-β（TGFβ）刺激细胞增殖和基质合成，但可逆地抑制碱性磷酸酶（Alpase）活性、X型胶原合成及随后钙化的终末分化诱导。当将甲状旁腺激素（PTH）添加到分化模型中时，Alpase的诱导和钙摄取均被可逆地抑制。相比之下，降钙素（CT）剂量依赖性地刺激Alpase活性和钙摄取。在FGF存在的情况下，骨形态发生蛋白（BMP）强烈刺激软骨细胞的DNA合成，并在生长阶段诱导软骨细胞快速成熟。此外，BMP在钙化阶段刺激多层软骨细胞中的Alpase活性。