甲状旁腺激素和降钙素对兔生长板软骨细胞培养物中碱性磷酸酶活性和基质钙化的影响。
Effects of parathyroid hormone and calcitonin on alkaline phosphatase activity and matrix calcification in rabbit growth-plate chondrocyte cultures.
作者信息
Kato Y, Shimazu A, Nakashima K, Suzuki F, Jikko A, Iwamoto M
机构信息
Department of Biochemistry, Faculty of Dentistry, Osaka University, Japan.
出版信息
Endocrinology. 1990 Jul;127(1):114-8. doi: 10.1210/endo-127-1-114.
The effects of PTH and calcitonin (CT) on the expression of mineralization-related phenotypes by chondrocytes were examined. In cultures of pelleted growth-plate chondrocytes. PTH caused 60-90% decreases in alkaline phosphatase activity, the incorporation of 45Ca into insoluble material, and the calcium content during the post-mitotic stage. These effects of PTH were dose-dependent and reversible. In contrast, CT increased alkaline phosphatase activity, 45Ca incorporation into insoluble material, and the calcium content by 1.4- to 1.8-fold. These observations suggest that PTH directly inhibits the expression of the mineralization-related phenotypes by growth-plate chondrocytes, and that CT has the opposite effects.
研究了甲状旁腺激素（PTH）和降钙素（CT）对软骨细胞矿化相关表型表达的影响。在团状生长板软骨细胞培养物中，PTH导致有丝分裂后阶段碱性磷酸酶活性、45Ca掺入不溶性物质以及钙含量降低60 - 90%。PTH的这些作用具有剂量依赖性且可逆。相比之下，CT使碱性磷酸酶活性、45Ca掺入不溶性物质以及钙含量增加了1.4至1.8倍。这些观察结果表明，PTH直接抑制生长板软骨细胞矿化相关表型的表达，而CT则具有相反的作用。
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