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[Brief counting method of airborne Cryptomeria japonica pollen by a combination of fluorescence antibody staining and flow cytometry].

作者信息

Takahashi Y, Yoshimura S, Masago H, Inouye K, Shirai T, Nagoya T, Sakaguchi M, Inouye S, Katagiri S

机构信息

Yamagata Prefectural Institute of Public Health.

出版信息

Arerugi. 1992 Nov;41(11):1611-3.

PMID:1492796
Abstract

Airborne pollens collected in a pollen collector (Virtual Impactor) was treated with a fluorescein isothiocyanate-labeled monoclonal antibody (KW-S10) which was strictly specific to Japanese cedar pollen antigen (Cry j I). Flow cytometric analysis revealed that the intensity of fluorescence of the pollen samples treated with the antibody was greater than that of non-treated reference pollen or the antibody treated Hinoki-cypress pollen. By use of this method, it may be possible to display the airborne pollen concentration within 20 min after sampling.

摘要

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Arerugi. 1992 Nov;41(11):1611-3.
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