使用稀释平板技术对影响霉菌计数的一些因素的比较研究。

Comparative study of some factors affecting enumeration of moulds using dilution plate techniques.

作者信息

Bragulat M R, Abarca M L, Bruguera M T, Cabañes F J

机构信息

Departamento de Patología y Producción Animales (Microbiología), Facultad de Veterinaria, Universidad Autónoma de Barcelona, Spain.

出版信息

Microbiologia. 1992 Nov;8(2):106-14.

PMID:1492950

Abstract

The influence of dilution plating technique, nature of diluent, culture media and incubation period on the enumeration of moulds have been studied. Three new culture media containing Auramine, Gentian Violet and Malachite Green respectively have been induced in this study. No significant differences were observed between results obtained after 3, 5 and 7 days of incubation. Significantly higher recoveries were obtained using the surface-spread method than pour plate method. Using the first technique no effect of diluent was observed, and among the different culture media studied higher counts were obtained with medium containing Auramine.

摘要

研究了稀释平板技术、稀释剂性质、培养基和培养时间对霉菌计数的影响。本研究诱导了三种分别含有金胺、龙胆紫和孔雀石绿的新型培养基。培养3天、5天和7天后获得的结果之间未观察到显著差异。使用表面涂布法获得的回收率明显高于倾注平板法。使用第一种技术未观察到稀释剂的影响，在所研究的不同培养基中，含金胺的培养基获得的计数更高。