串珠镰刀菌选择性培养基的研制。
Development of a selective culture medium for Fusarium moniliforme.
作者信息
Castellá G, Bragulat M R, Rubiales M V, Cabañes F J
机构信息
Departament de Patologia i Producció Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, España.
出版信息
Microbiologia. 1997 Dec;13(4):493-8.
Nash and Snyder medium and malachite green agar 2.5 ppm medium, a new selective culture medium designed in our laboratory, were challenged with pure cultures of Fusarium moniliforme strains and two different mixed-conidium suspensions, which included rapidly spreading fungi, for their utility in the isolation and enumeration of F. moniliforme. From the results of this comparative study, malachite green agar 2.5 ppm allowed only the selective growth of F. moniliforme whereas Nash and Snyder medium allowed both the growth of F. moniliforme and other species not belonging to Fusarium spp. The enumeration of F. moniliforme propagules was similar in both culture media.
纳什和斯奈德培养基以及我们实验室设计的一种新型选择性培养基——2.5 ppm孔雀石绿琼脂培养基，用串珠镰刀菌菌株的纯培养物以及两种不同的混合分生孢子悬浮液（其中包括快速蔓延的真菌）进行测试，以评估它们在分离和计数串珠镰刀菌方面的效用。从这项比较研究的结果来看，2.5 ppm孔雀石绿琼脂培养基仅允许串珠镰刀菌选择性生长，而纳什和斯奈德培养基既允许串珠镰刀菌生长，也允许其他非镰刀菌属的物种生长。两种培养基中串珠镰刀菌繁殖体的计数结果相似。
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