Krieg A M, Gourley M F, Klinman D M, Perl A, Steinberg A D
Department of Internal Medicine, University of Iowa, Iowa City 52242.
AIDS Res Hum Retroviruses. 1992 Dec;8(12):1991-8. doi: 10.1089/aid.1992.8.1991.
This study examines the expression of human endogenous retroviral or retroviral-like (ERV) sequences in peripheral blood mononuclear cells (PBMC). Probes to 12 human ERV were used in Northern analyses of 38 patients with autoimmune muscle diseases and 31 blood donor controls. All patients and controls expressed multiple classes of ERV RNA. This expression was quite heterogenous: for each of the nine ERV classes for which expression was detected, some individuals showed high RNA levels whereas others showed low levels. ERV expression was independent of disease and autoantibody production. Statistical analysis of densitometric data indicated that expression of several classes of ERV was coordinately regulated. ERV expression in individual patients showed coordinate fluctuations with time. These studies demonstrate the heterogeneity and coordinate regulation of human ERV expression. To evaluate whether ERV expression might be affected by lymphocyte activation, PBMC were cultured with or without lymphocyte mitogens before RNA extraction. These studies demonstrated complex changes in ERV expression after lymphocyte activation. Murine ERV have several immunoregulatory activities. If human ERV have analogous effects, their heterogeneous expression and association with lymphocyte activation may have important biologic consequences.
本研究检测了人内源性逆转录病毒或逆转录病毒样（ERV）序列在外周血单个核细胞（PBMC）中的表达。利用针对12种人ERV的探针，对38例自身免疫性肌肉疾病患者和31名献血者对照进行了Northern分析。所有患者和对照均表达多种类别的ERV RNA。这种表达非常异质性：对于检测到表达的九种类别的ERV中的每一种，一些个体显示出高RNA水平，而另一些个体则显示出低水平。ERV表达与疾病和自身抗体产生无关。对光密度数据的统计分析表明，几种类别的ERV表达受到协同调节。个体患者中的ERV表达随时间呈现协同波动。这些研究证明了人ERV表达的异质性和协同调节。为了评估ERV表达是否可能受淋巴细胞活化的影响，在提取RNA之前，将PBMC在有或无淋巴细胞有丝分裂原的情况下进行培养。这些研究证明了淋巴细胞活化后ERV表达的复杂变化。鼠类ERV具有多种免疫调节活性。如果人类ERV具有类似作用，它们的异质性表达以及与淋巴细胞活化的关联可能具有重要的生物学意义。
