Joling P, van Wichen D F, Parmentier H K, Biberfeld P, Böttiger D, Tschopp J, Rademakers L H, Schuurman H J
Department of Pathology, University Hospital, Utrecht, The Netherlands.
AIDS Res Hum Retroviruses. 1992 Dec;8(12):2021-30. doi: 10.1089/aid.1992.8.2021.
We studied follicles in sections of lymph nodes and spleen from cynomolgus monkeys (Macaca fascicularis) after infection with simian immunodeficiency virus (SIVsm), by (immuno)histology and (immunogold) electron microscopy. Also isolated follicular dendritic cells (FDC) were investigated. Histology showed ranged from follicular hyperplasia to follicle fragmentation. FDC showed desmin and vimentin, characteristic of mesenchymal cells. Except for two animals who got experimental chemotherapy in the first postinfection period, the cells expressed SIV gag p28 protein. Electron microscopy showed SIVsm-like particles in the germinal centers. A number of cell types in the germinal center, including FDC, showed tubuloreticular structures, indicative of alpha-interferon synthesis during an antiviral response. In immunogold electron microscopy, SIV p28 label was observed on the surface of FDC, on SIVsm-like particles, and in the cytoplasm of macrophages. A relatively high density of CD8-positive cells (T cytotoxic-suppressor phenotype) was observed around and in germinal centers, especially areas depleted of FDC. Cells immunoreactive for serine esterase granzyme-B, a protein occurring in granules of cytotoxic cells, occurred around germinal centers, but not in germinal centers at areas where FDC and SIV p28 label localized. This argues against a role of cytotoxic T cells in mediating follicle destruction.
我们通过（免疫）组织学和（免疫金）电子显微镜，研究了感染猿猴免疫缺陷病毒（SIVsm）后的食蟹猴（猕猴）淋巴结和脾脏切片中的滤泡。还对分离出的滤泡树突状细胞（FDC）进行了研究。组织学显示从滤泡增生到滤泡破碎不等。FDC显示结蛋白和波形蛋白，这是间充质细胞的特征。除了两只在感染后第一个时期接受实验性化疗的动物外，这些细胞表达SIV gag p28蛋白。电子显微镜显示生发中心有类似SIVsm的颗粒。生发中心的许多细胞类型，包括FDC，都显示出管状网状结构，这表明在抗病毒反应期间有α-干扰素合成。在免疫金电子显微镜下，在FDC表面、类似SIVsm的颗粒上以及巨噬细胞的细胞质中观察到SIV p28标记。在生发中心及其周围观察到相对高密度的CD8阳性细胞（细胞毒性抑制性T细胞表型），特别是在FDC缺失的区域。对丝氨酸酯酶颗粒酶B有免疫反应的细胞，一种存在于细胞毒性细胞颗粒中的蛋白质，出现在生发中心周围，但在FDC和SIV p28标记定位的生发中心区域未出现。这表明细胞毒性T细胞在介导滤泡破坏中不起作用。
