碱性成纤维细胞生长因子（bFGF）在星形胶质细胞和海马细胞培养物中诱导其自身的基因表达。
bFGF induces its own gene expression in astrocytic and hippocampal cell cultures.
作者信息
Flott-Rahmel B, Gerdes W, Pechan P A, Brysch W, Schlingensiepen K H, Seifert W
机构信息
Department of Neurobiology, Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany.
出版信息
Neuroreport. 1992 Dec;3(12):1077-80. doi: 10.1097/00001756-199212000-00011.
Basic FGF mRNA induction by bFGF was investigated in cell cultures from rat brain, i.e. postnatal day 2 cortex and embryonic day 18 hippocampus. In situ hybridization shows that after bFGF treatment (10(-10) M) for 14 h neurones and glial cells show a remarkable increase in bFGF mRNA production. Incubation of astrocytes with antisense bFGF phosphorothioate oligodeoxynucleotides (bFGF-PTOs) resulted in an inhibition of both bFGF induced and serum induced proliferation. The results indicate that bFGF is capable of inducing its own mRNA production. This induction, i.e. new synthesis of bFGF mRNA, seems to be essential for the mitogenic effect of both bFGF and serum components.
在大鼠脑的细胞培养物中，即出生后第2天的皮层和胚胎第18天的海马体中，研究了碱性成纤维细胞生长因子（bFGF）对碱性成纤维细胞生长因子信使核糖核酸（Basic FGF mRNA）的诱导作用。原位杂交显示，在10⁻¹⁰M的bFGF处理14小时后，神经元和神经胶质细胞中bFGF mRNA的产生显著增加。用反义bFGF硫代磷酸酯寡脱氧核苷酸（bFGF-PTOs）孵育星形胶质细胞，导致bFGF诱导的和血清诱导的增殖均受到抑制。结果表明，bFGF能够诱导其自身mRNA的产生。这种诱导，即bFGF mRNA的新合成，似乎对bFGF和血清成分的促有丝分裂作用至关重要。
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