Cooley M A
Scand J Immunol. 1978;7(5):371-80. doi: 10.1111/j.1365-3083.1978.tb00467.x.
The effect of intravenous injection of 10(6) BALB/c spleen cells into C57B1/6J recipients was assayed by both mixed lymphocyte culture (MLC) of recipient lymphocytes, and by grafting donor (BALB/c) thyroids into recipient mice. It was observed that a single intravenous injection produced depression of proliferative and cytotoxic T cell responses in MLC of spleen, lymph node and peripheral blood lymphocytes of the recipients. This effect was specific for the sensitizing genotype (MLC of recipient and third-party CBA/H lymphocytes was unaffected), and persisted for several days after sensitization. The pattern of this diminished response suggested that the effect was due to a combination of recruitment of reactive lymphocytes from peripheral lymphoid populations, and the generation of alloantigen (H-2?)-specific suppressor T cells in the spleen. In contrast to these findings in vitro, a similar sensitization led only to accelerated rejection of thyroid allografts in vivo.
通过受体淋巴细胞的混合淋巴细胞培养（MLC）以及将供体（BALB/c）甲状腺移植到受体小鼠体内，检测了向C57B1/6J受体静脉注射10(6)个BALB/c脾细胞的效果。观察到单次静脉注射会使受体脾、淋巴结和外周血淋巴细胞的MLC中增殖性和细胞毒性T细胞反应受到抑制。这种效应对于致敏基因型具有特异性（受体与第三方CBA/H淋巴细胞的MLC未受影响），并且在致敏后持续数天。这种反应减弱的模式表明，该效应是由于外周淋巴群体中反应性淋巴细胞的募集以及脾脏中同种异体抗原（H-2?）特异性抑制性T细胞的产生共同作用的结果。与这些体外研究结果相反，类似的致敏在体内仅导致甲状腺同种异体移植的排斥加速。
