Cioli D, Pica-Mattoccia L, Moroni R
Institute of Cell Biology, Rome, Italy.
Exp Parasitol. 1992 Dec;75(4):425-32. doi: 10.1016/0014-4894(92)90255-9.
Individual schistosomes of an hycanthone/oxamniquine-sensitive strain were crossed with individual schistosomes of the opposite sex and belonging either to the same sensitive population or to a different strain which exhibited high resistance to the two drugs. Schistosome crosses were performed by transfer of single worm pairs into the mesenteric veins of mice and the drug sensitivity/resistance of individual progeny worms was assessed using an in vitro test. Drug resistance behaved as an autosomal recessive trait, as shown by the results of the F1 and F2 generation and of the backcrosses. Drug-resistant worms appeared to be slightly less viable than their sensitive counterpart at all stages of the life cycle. The results are relevant for an interpretation of drug resistance and drug mechanisms and the approach used in this study may be applicable to different genetic markers in schistosomes.
将对海恩酮/奥沙尼喹敏感品系的单个血吸虫与异性个体进行杂交，这些异性个体要么属于同一敏感群体，要么属于对这两种药物具有高度抗性的不同品系。通过将单个虫对转移到小鼠的肠系膜静脉中来进行血吸虫杂交，并使用体外试验评估单个子代虫的药物敏感性/抗性。如F1和F2代以及回交结果所示，耐药性表现为常染色体隐性性状。在生命周期的所有阶段，耐药虫似乎比其敏感对应物的生存能力略低。这些结果对于解释耐药性和药物作用机制具有重要意义，本研究中使用的方法可能适用于血吸虫的不同遗传标记。
