Texas Biomedical Research Institute, San Antonio, Texas 78227, USA.
University of Texas Health, San Antonio, Texas 78229, USA.
Trends Parasitol. 2018 Nov;34(11):982-996. doi: 10.1016/j.pt.2018.08.001. Epub 2018 Aug 24.
Linkage mapping - utilizing experimental genetic crosses to examine cosegregation of phenotypic traits with genetic markers - is now 100 years old. Schistosome parasites are exquisitely well suited to linkage mapping approaches because genetic crosses can be conducted in the laboratory, thousands of progeny are produced, and elegant experimental work over the last 75 years has revealed heritable genetic variation in multiple biomedically important traits such as drug resistance, host specificity, and virulence. Application of this approach is timely because the improved genome assembly for Schistosoma mansoni and developing molecular toolkit for schistosomes increase our ability to link phenotype with genotype. We describe current progress and potential future directions of linkage mapping in schistosomes.
连锁映射——利用实验性遗传杂交来检查表型特征与遗传标记的共分离——已经有 100 年的历史了。血吸虫寄生虫非常适合连锁映射方法，因为可以在实验室中进行遗传杂交，产生数千个后代，并且在过去的 75 年中进行的巧妙的实验工作揭示了多种与医学相关的特征中的可遗传遗传变异，如药物抗性、宿主特异性和毒力。这种方法的应用是及时的，因为曼氏血吸虫的基因组组装得到了改进，并且血吸虫的分子工具包也在不断发展，这提高了我们将表型与基因型联系起来的能力。我们描述了在血吸虫中连锁映射的当前进展和潜在的未来方向。
